How does increasing the substrate concentration affect the rate of an enzyme-catalyzed reaction?
The rate decreases as the active sites become blocked.
The rate increases indefinitely.
The rate increases until all active sites are saturated.
The rate remains constant regardless of concentration.
📝 Detailed explanation
As substrate concentration increases, more active sites are occupied. Eventually, every enzyme molecule is working at maximum capacity (saturation), and the rate levels off ($V_{max}$).
