Which of the following is the correct procedure for testing a sample for a non-reducing sugar (like sucrose)?

  1. Add Benedict's reagent and heat.
  2. Add Iodine solution and observe color.
  3. Boil with dilute HCl, neutralize with alkali, then add Benedict's and heat.
  4. Mix with ethanol and pour into water.

📝 Batafsil tushuntirish

Sucrose must first be hydrolyzed into glucose and fructose (reducing sugars) by acid before the Benedict's test can give a positive result.
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